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KMID : 1134820140430121858
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 12 p.1858 ~ p.1864
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Isolation and Identification of α-Glucosidase Inhibitory Compounds, Hyperoside, and Isoquercetin from Eleutherococcus senticosus Leaves
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Lee Ki-Yeon
Hong Soo-Young
Jeong Hye-Jeong
Lee Jae-Hyoung
Lim Sang-Hyun
Heo Nam-Ki
Kang Song-Mun
Kim Hee-Yeon
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Abstract
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In the present investigation, the anti-diabetic potential of 80% ethanol extract of Eleutherococcus senticosus leaves (EEES) was examined based on α-glucosidase inhibitory activities. EEES was sequentially fractionated with n-hexane, chloroform, ethyl acetate (EtAOc), n-butanol, and H2O. Of the various fractions, EtAOc fraction effectively inhibited α-glucosidase activity by 68.05%. Therefore, EtAOc fraction was selected for further isolation and identification studies. EtAOc fraction was separated by medium pressure liquid chromatography with silica and ODS gel to yield eight fractions (EAA～EAH). Based on the results of α-glucosidase inhibitory activity, EAH fraction was re-chromatographed to yielded four more fractions (EAHA～EAHD). Of these, EAHC fraction showed higher α-glucosidase inhibitory activity of 93.60%. EAHC fraction was re-chromatographed and yielded EAHCA and EAHCB fractions. Further, identification and chemical structures of these two fractions were analyzed using 1H-NMR, 13C-NMR, and mass spectra data. Based on the results of the spectral data, the isolated compounds were identified as hyperoside and isoquercetin. Results of the present study indicate that the isolated compounds, hyperoside, and isoquercetin from leaves of E. senticosus could be used for the development of new anti-diabetic drugs.
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KEYWORD
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Eleutherococcus senticosus, α-glucosidase inhibitor, hyperoside, isoquercetin
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